IMAGING ENDOTHELIAL CALCIUM ACTIVITY AND BLOOD FLOW IN VIVO

Left: Video showing GCaMP8 reporting calcium signals in endothelial cells (green), throughout the vasculature in the barrel cortex in an anesthetized naïve animal. Middle: The same conditions recorded in a mouse after 1 day of enrichment through living in a bead cage. Right: The same conditions recorded in a mouse after 7 days of enrichment.